Thursday, September 16, 2021

Commensal Bacterial Antibiosis in the Gut of the Estuarine Water Fish ‘Chelon parsia'

 A new strain Bacillus subtilis PP2 was discovered after screening the gut flora of the estuary water fish 'Chelon parsia' for the presence of potential antibiotic makers.Further research into the active principle revealed that the molecule is a peptide class antibacterial agent with a m/z of 1006.66.

The modification of culture conditions with enhanced nitrogen and carbon supply combination and concentration had a significant impact on bacterial growth and peptide production.The partially described peptide antibiotic was discovered to be a broad-spectrum antifungal agent capable of acting against a variety of fungal diseases during the early stages of pharmacological intervention.



Wednesday, September 1, 2021

#Brettanomyces #bruxellensis is a major source of wine deterioration in wineries all over the world.


Dekkera/Brettanomyces bruxellensis is now the yeast responsible for the most dreaded phenolic off-flavor in red wine. Discoveries about its initial physiological and genetic nature have recently piqued the interest of the wider scientific community, not simply the oenology community.Over the last three years (2007–2009), B. bruxellensis has accounted for almost 30% of all records published on this topic since 1960. Recent research findings discussed in this chapter have greatly improved understanding of this unique biological system, its ecology, the major phenotypic and genotypic features distinguishing different strains, the phenolic off-flavor pathway, and methods for detecting, preventing, and correcting B. bruxellensis spoilage faults.

Monday, August 23, 2021

The Sputum Microbiome in Pulmonary #Tuberculosis and Its Relationship to Disease Symptoms

 Every day, over 27,000 individuals become ill with tuberculosis (TB), with 4,000 dying as a result of the disease. Pulmonary tuberculosis is the most common clinical form of tuberculosis, and it affects the lungs in a wide range of people.

Immunomodulation by an interaction of host-, environment-, and pathogen-associated variables explains some of this variation. Microbial communities in the host's airways have immunomodulatory effects, but it is unclear whether the inter-individual diversity of these microbial communities is related to the heterogeneity of pulmonary tuberculosis.

This hypothesis was addressed by analysing the microbial composition in the sputum of 334 Tanzanian TB patients and assessing its correlation with three characteristics of clinical manifestations:Mycobacterial load in sputum, severe clinical symptoms, and chest x-ray (CXR) findings Compositional data analysis of taxonomic profiles based on 16S-rRNA gene amplicon sequencing and whole metagenome shotgun sequencing, as well as graph-based inference of microbial connections, revealed that Streptococcus and two anaerobes controlled the airway microbiome of TB patients.

Monday, August 16, 2021

Antibiotic Resistance Transfer in Bacteria in the Gut


Horizontal gene transfer across microorganisms in the gastrointestinal (GI) tract was investigated. 
The rise of antibiotic-resistant strains and treatment failures of bacterial illnesses have raised public awareness of antibiotic use in recent decades.The use of broad-spectrum antibiotics places selection pressure on the bacterial flora, boosting the establishment of multiresistant bacteria, resulting in a vicious circle of treatment and the emergence of new antibiotic resistant bacteria.
The human gastrointestinal tract is large reservoir of bacteria that has the ability to receive and disseminate antibiotic resistance genes.The increased usage of fermented foods and probiotics as food supplements and health-promoting goods containing huge volumes of bacteria acting as either donors or recipients of antibiotic resistance genes in the human GI tract also adds to the establishment of antibiotic-resistant strains.This research examines the evaluation of antibiotic resistance gene transfer in the stomach.

Tuesday, August 10, 2021

Vancomycin-Resistant Enterococci (VRE) Colonization in #HIV-Infected Patients Attending ART Clinic

 Background. Enterococci that invade the intestines of immunocompromised people are a major source of nosocomial infections. In the current study area, data on the frequency of vancomycin-resistant Enterococci (VRE), as well as its antimicrobial susceptibility patterns and related variables, are rare.

As a result, the purpose of this study was to determine the prevalence of VRE colonisation among HIV-infected patients attending ART clinics at West Amhara Government Hospitals. Methods. From 1 February to 31 May 2017, a cross-sectional study was undertaken. 

Friday, August 6, 2021

The #MicrobialCommunity of Poultry Farm Waste and Its Role in the Production of Hydrogen Sulfide

 Despite its importance in the generation of volatile odorants and disagreeable odours, the microbiota of chicken litter has received little attention. One of the main components of the odour is hydrogen sulphide, which can be produced through microbial sulphate reduction. The microbial community composition of poultry factory waste was examined.The samples were taken at the Mezheninovskaya huge poultry farm, located 15 kilometres from Tomsk, Russia. The microbial community's 16S rRNA gene profiling indicated sulfate-reducing Desulfovibrionaceae. After incubating the trash in a medium containing sulphate and lactate, both their community share and sulphide production increased.Gypsum and feed additives supplied as sulphates, such as lysine sulphate, are potential sulphate sources in trash.

Friday, July 30, 2021

An open-label randomised experiment found that oral hydroxychloroquine plus povidone-iodine throat spray had a positive influence on #COVID19 prevention.

 Non-pharmacological interventions (NPIs) are the only established strategies to reduce COVID-19 transmission until mass immunisation is successfully adopted globally (Lee et al., 2020, Sun et al., 2020, Chen et al., 2020, Wiersinga et al., 2020).Most countries have taken stringent measures to restrict physical movements, such as blocking borders, schools, restaurants, pubs, and religious activities, while targeted quarantine restricts movement of identified contacts (Lee et al., 2020, Sun et al., 2020, Chen et al., 2020).Despite this, the outbreak has been difficult to contain, since additional case clusters have emerged and even increased in number even after the initial quarantine measures were implemented.

Tuesday, July 27, 2021

Hospital-acquired infections in Ukraine

 Hospital-Acquired Infection affects around 15.0 percent of all hospitalized patients, according to the WHO (HAIs). HAIs are found in 13% to 2% of hospitalized patients, according to data from the United States and Europe. In Ukraine, there are 5-7.000 documented instances of HAIs each year, with an additional 500.000 unregistered cases. One option for preventing HAIs is to implement a control strategy in the intensive care unit (ICU), where last-line antibiotics are administered.

Friday, July 23, 2021

Probiotics alter intestine immunological and serotonergic gene expression in mice in a site-specific manner.

 Probiotic microorganisms may benefit the host by influencing a variety of physiological processes, the nature of which and the underlying mechanisms are still completely unknown. Animal models are a one-of-a-kind tool for comprehending the complexities of interactions between probiotic microbes, the gut microbiota, and the host. This pilot study was conducted in this regard.We examined the effects of three different probiotic bacterial strains (Bifidobacterium bifidum MIMBb23sg, Lactobacillus helveticus MIMLh5, and Lacticaseibacillus paracasei DG) on three unique mouse intestinal locations after 5 days of treatment (ileum, cecum and colon). All probiotics colonised
the cecum and colon preferentially.

Friday, July 16, 2021

#BoneTissue #Engineering

 Scaffolds of Vascularized Bacterial Nanocellulose and Hydroxyapatite for #BoneTissue #Engineering

The Allure of Cytokine #Immunological Science

Today, it is known that all human biological functions are under two fundamental regulatory systems, consisting of the endocrine system and the cytokine network. Moreover, it has been shown that the cytokines released from the activated immune cells do not influence only immune functions, but also the whole biological system, including the various metabolic activities, the cardiovascular system, and the functionless of the neuroendocrine system itself. Unfortunately, despite the well-demonstrated importance of cytokines in maintaining the status of health, from a clinical point of view the routine evaluation of the cytokine system still remains unconsidered to establish the status of health, since it is investigated only in severe conditions, such as septic shock, disseminated intravascular coagulation and respiratory distress, which have been demonstrated to be due to an abnormal endogenous production of inflammatory cytokines, namely IL-6, TNF-alpha, and IL-1 beta.

Friday, July 2, 2021

Microbiology & Infectious Diseases


In mycobacteria and other human diseases, central carbon metabolism and metal homeostasis interact.